Description
The risk of infection from pathogens and dissemination of resistant genes present in greywater is considered to be the most significant human health risk associated with greywater use. Thus this project was aimed at detecting and enumerating antibiotic resistant genes (ARGs) in domestic greywater systems, and to assess whether these systems can support the growth and proliferation of resistant organisms. The specific objectives were as follows: i) Determine whether domestic greywater is a source of antibiotic resistance genes and pathogenic organisms; ii) Enumeration of ARGs in various greywater sources using qPCR; iii) Water analysis to measure the levels of antibiotics present in greywater; iv) Development of novel FACS assays to screen for ARGs from environmental samples; v) Screen for the presence of integrons as markers of horizontal gene transfer.
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